Marna Wet Lab / Flow Lab / Mesocosm Lab

Equipment

Seawater Intake

• Intake line: 4” HDPE, 220m long, 21m depth, elevated 2m off the seafloor

  • ¼” basket strainers, mounted in-line, just upstream of the intake pumps

• Two x 3 HP impeller pumps, variable speed

  • Min flow rate: 150 LPM

  • Max flow rate: 360 LPM

• Water storage

  • 2 x 4350 L raw water tanks

    • Usable volume: 5120 L total (due to the location of the filter pump intake and the tank overflow openings)

  • 1 x 1820 L raw water tank – primary for the sea tables

    • Usable volume: 1306 L

Water Treatment System

• Filter Pumps: Two x 3 HP impeller pumps, variable speed

  • Max flow rate: 250 LPM

• Physical filtration

  • AZUD pre-filter system with 20µm disks, self-cleaning

  • Two parallel banks of 25, 10, 5, and 1µm polypropylene bag filters

• UV treatment

  • 4 x 320 W lamps, 180mJ/cm² at 191 LPM

• Treated water storage

  • 2 x 4350 L water tanks

    • Usable volume: 5120 L total (due to the location of the lab pump intake and the tank overflow openings)

• Lab supply pumps: Two x 1 HP impeller pumps

  • Max flow rate: 136 LPM

  • Total intake flow is split between the Wet Lab and the sea table supplies, so the flow rate available to the lab is limited by the flow to the sea tables

• Wastewater treatment

  • 1.0 m³ concrete settling sump, divided into 3 sections by baffles 

  • 4 x 57 W UV lamps

Mesocosms

• 40 x 340L tanks with independent temperature control (216L experimental chamber) 

• pH and temperature monitoring

• Recirculating and flow through options

• Individual UV treatment

• LED lights and photoperiod options

Heating and Cooling Systems

• Heat and cool the mesocosm system with individual titanium heat exchanger coils in every tank

• Temperature range from 8-30oC

• Glycol used for heating and cooling

  • Hot Supply

    • One heat pump (52000 BT/hr) and two auxiliary propane heaters

    • Maintained at 45oC

  • Cold Supply

    • Three heat pumps (3.35 ton each)

Maintained at 2oC

Mass Flow Controllers and Air Delivery

• Alicat Scientific Mass Flow Controllers for air and CO2

• Ability to offer up to 4 treatments with CO2

• Static or dynamic treatments

  • Dynamic treatments can Include simulating time series data and future climate predictions

• Airstones bubble into every mesocosm, gas delivery at 8 LPM

• Rotary compressor with 340 LPM capacity

Sensor Network and Monitoring

• Monitoring tank temperature and pH of mesocosms

• Zabbix system tracking pumps, holding tanks levels and an array of other equipment to provide real time monitoring

Burke-o-lator

• Monitoring incoming lab water

• Testing water from the mesocosm treatments and chemical manipulation system

• Processing samples from all in house experiments to analyze carbonate chemistry

Chemical Manipulation System

• Three treatment channels

• Using acids and bases to create treatments

• Flow through chamber capabilities for larval experiments

Culture Systems

• Larval Rearing

  • A variety of equipment to rear larvae for experimental purposes including upwelling and rolling systems, water baths and specialized containers for holding larvae or small organisms in experimental systems

• Microalgae culture

  • 2 x 1250L Industrial Plankton Photobioreactors for growing microalgae

  • Culture collection of 12 microalgae strains

• Macroalgae culture

  • Germplasm collection of Nereocystis luetkeana

  • Ability to culture other species if interested

Animal Holding

• Various sized sea tables for hosting fish and marine invertebrates

  • Effluent treated by UV 

• Shellfish raft for holding juvenile and adult shellfish